EDIM technology is a new diagnostic procedure, assembled and used in PanTum Detetct, which utilizes mechanisms of the immune system in order to achieve a high diagnostic accuracy.
EDIM technology examines macrophages to detect antigens that have been phagocytosed by these immune cells previously – this is why we describe the technology as a biological biopsy. For this purpose, CD14 and CD16 surface antibodies are used, which bind specifically to the surface of the cells. The cells are then permeabilised briefly to induce further antibodies to enter the cells. These antibodies are directed against the antigens which are detected by the method. For example, in the case of PanTum Detect, TKTL1 and Apo10 are inserted into the cells.
The cell structures on the surface of cells can be recognised very efficiently in blood samples with dye-labelled antibodies and a subsequent detection of these dyes by means of laser beams using the method of flow cytometry. This makes it possible to recognise, count and further characterise macrophages in the blood. In this way, signals are also detected which are derived from bound antibodies in the cell interior. This makes it possible to detect what the phagocyte has detected and eliminated by phagocytosis. If for example in the case of a tumour disease there are tumour cells in the organism, the system detects signals from the dyes that are coupled to the TKTL1 and Apo10 antibodies.